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Published on: April 11, 2017
A novel highly selective FRET sensor for Fe(III) and DFT mechanistic evaluation
Ketsarin Chantarasunthon1, Malinee Promkatkaew1, Patthreera Waranwongcharoen1
1Department of Basic Science and Physical Education, Faculty of Science at Sriracha, Kasetsart University Sriracha Campus, Chonburi 20230, Thailand.
Abstract:
A novel FRET-based sensor has been designed and developed through the conjugation of naphthyl and rhodamine via propylamine spacer, Naph-Rh. The naphthyl moiety serves as a FRET donor due to its emission spectrum overlapping with the rhodamine B absorption band. Naph-Rh exhibited a selectivity for sensing Fe3+ over other metal ions with a visual color change and fluorescent enhancement. The ratio of the Naph-Rh and Fe3+ was determined to be 1:1 based on Job's plot analysis with a detection limit of 83 nM. The probe exhibited a linear response to Fe3+ in the range of 0-120 μM. Furthermore, the density functional theory (DFT) calculations of Naph-Rh were carried out to rationalize the design and portray the plausible Fe3+ sensing mechanism.
