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Related Experiment Video

Updated: Aug 23, 2025

Direct Tracheal Instillation of Solutes into Mouse Lung
04:50

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An optimized method for intratracheal instillation in mice.

Yi Zeng1, Huidong Jin1, Jia Wang1

  • 1Department of Environmental Health, College of Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, China.

Journal of Pharmacological and Toxicological Methods
|November 2, 2022
PubMed
Summary
This summary is machine-generated.

This study presents an improved non-invasive intratracheal instillation method for respiratory tract exposure in mice. The validated technique ensures accuracy and reliability, supporting animal welfare and research in toxicology and environmental science.

Keywords:
Intratracheal instillationIntubationMethodsNon- invasive

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Area of Science:

  • Toxicology
  • Environmental Science
  • Respiratory Research

Background:

  • Non-invasive intratracheal instillation is crucial for respiratory tract exposure in research.
  • A lack of standardized operating procedures can impact the reliability of this method.
  • Ensuring accuracy is vital for animal models, especially in chronic exposure studies.

Purpose of the Study:

  • To improve and validate a non-invasive intratracheal instillation technique.
  • To assess the accuracy and reliability of the enhanced method.
  • To demonstrate the method's utility in animal models for respiratory exposure studies.

Main Methods:

  • Adult female BALB/c mice were instilled with ink solution, normal saline, or PM2.5 suspension.
  • Ink distribution was assessed anatomically post-instillation.
  • PM2.5 distribution and lung injury were evaluated via pathological staining after 24 hours.

Main Results:

  • Ink was confined to the lungs, with no presence in the gastrointestinal tract, confirming targeted delivery.
  • PM2.5 particles were observed in the terminal bronchioles and alveolar spaces.
  • Acute lung injury was evident following PM2.5 exposure.

Conclusions:

  • The improved non-invasive intratracheal instillation method is accurate and reliable.
  • This technique minimizes harm to animals, aligning with 3R animal welfare principles.
  • The method is suitable for establishing mouse models for short-term and long-term respiratory tract exposure.