Melatonin delays ABA-induced leaf senescence via H₂ O₂ -dependent calcium signalling

Precocious leaf senescence can reduce crop yield and quality by limiting the growth stage. Melatonin has been shown to delay leaf senescence; however, the underlying mechanism remains obscure. Here, we show that melatonin offsets abscisic acid (ABA) to protect photosystem II and delay the senescence of attached old leaves under the light. Melatonin induced H₂ O₂ accumulation accompanied by an upregulation of melon respiratory burst oxidase homolog D (CmRBOHD) under ABA-induced stress. Both melatonin and H₂ O₂ induced the accumulation of cytoplasmic-free Ca²⁺ ([Ca²⁺ ]_cyt ) in response to ABA, while blocking of Ca²⁺ influx channels attenuated melatonin- and H₂ O₂ -induced ABA tolerance. CmRBOHD overexpression induced [Ca²⁺ ]_cyt accumulation and delayed leaf senescence, whereas deletion of Arabidopsis AtRBOHD, a homologous gene of CmRBOHD, compromised the melatonin-induced [Ca²⁺ ]_cyt accumulation and delay of leaf senescence in Arabidopsis under ABA stress. Furthermore, melatonin, H₂ O₂  and Ca²⁺ attenuated ABA-induced K⁺ efflux and subsequent cell death. CmRBOHD overexpression and AtRBOHD deletion alleviated and aggravated the ABA-induced K⁺ efflux, respectively. Taken together, our study unveils a new mechanism by which melatonin offsets ABA action to delay leaf senescence via RBOHD-dependent H₂ O₂ production that triggers [Ca²⁺ ]_cyt accumulation and subsequently inhibits K⁺ efflux and delays cell death/leaf senescence in response to ABA.