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Monitoring of Post-Brain Injuries By Measuring Plasma Levels of Neuron-Derived Extracellular Vesicles
Naoshi Hotta1, Takahiro Tadokoro1, John Henry2
1Department of Anesthesiology, National Cerebral and Cardiovascular Center, Osaka, Japan.
Biomarker Insights
|November 3, 2022
Summary
A new blood test measures neuron-derived extracellular vesicles (NDE) to assess brain injury. NDE levels, while varying between individuals and decreasing with age, are stable within individuals and can indicate post-surgery complications like delirium.
Area of Science:
- Neuroscience
- Biomarker Discovery
- Extracellular Vesicles
Background:
- Extracellular vesicles (EV) in blood reflect nervous system status.
- Neuron-derived EVs (NDE) show potential as brain injury biomarkers.
Purpose of the Study:
- Establish a reliable method for measuring plasma NDE.
- Investigate NDE levels in athletes and post-surgery patients.
- Assess NDE as a potential indicator of brain injury and postoperative delirium.
Main Methods:
- Developed a sandwich immunoassay to quantify neuron-derived EVs (CD171+ CD9+).
- Collected plasma from athletes (cross-country, football, soccer, rugby) and patients undergoing total aortic arch replacement (TAR).
- Analyzed NDE levels concerning age, individual stability, athletic activity, and postoperative delirium (POD).
Main Results:
- The NDE assay demonstrated specificity, linearity, and reproducibility.
- NDE levels exhibited significant inter-individual variability and an age-dependent decrease.
- NDE levels were stable within individuals over time but increased post-TAR, correlating with POD development.
Conclusions:
- A validated immunoassay can measure plasma NDE.
- NDE levels require normalization for biomarker studies due to individual variability and age effects.
- NDE assay shows promise for monitoring brain injury and predicting postoperative delirium.
