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Prostate cancer-associated urinary proteomes differ before and after prostatectomy.

Yan Feng1,2, Shengzhi Liu2, Rongrong Zha1,2

  • 1Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin, China.

Therapeutic Advances in Medical Oncology
|November 3, 2022
PubMed
Summary

Prostatectomy alters urine proteins, revealing tumor-suppressive profiles. Post-surgery urine inhibits cancer cell growth, identifying key proteins like prostasin and nectin 2 as potential tumor suppressors.

Keywords:
angiogeninnectin 2nidogen 1prostasinprostate cancerprostatectomyurine

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Area of Science:

  • Urology
  • Oncology
  • Proteomics

Background:

  • Urinary proteomes offer insights into various disorders, including cancer.
  • Tumor-modifying proteins in urine can serve as diagnostic markers and indicators of treatment efficacy.

Purpose of the Study:

  • To investigate changes in urinary proteomes of prostate cancer patients before and after prostatectomy.
  • To identify specific tumor-modifying proteins and their roles in cancer progression and suppression.

Main Methods:

  • Analysis of urinary proteomes from prostate cancer patients pre- and post-prostatectomy.
  • Protein array analysis to evaluate tumor-promoting cytokines.
  • Mass spectrometry-based global proteomics to identify tumor-suppressing proteins.

Main Results:

  • Prostatectomy significantly altered urinary proteome profiles.
  • Post-prostatectomy urine exhibited reduced tumorigenic behavior in prostate and breast cancer cells.
  • Key findings include reduced angiogenin and elevated extracellular prostasin (PRSS8), nectin 2 (PVRL2), and nidogen 1 (NID1), which suppressed tumor growth and induced apoptosis.

Conclusions:

  • Prostatectomy induces significant changes in urinary proteomes, yielding tumor-suppressive profiles.
  • This study highlights the dynamic nature of urinary proteomes and identifies potential urinary tumor suppressors.
  • Urinary proteomic analysis presents a novel strategy for predicting tumor suppressors.