Modulation of male mouse sniff, attack, and mount behaviors by estrous cycle-dependent urinary cues

Modulation of male urogenital sniff, attack, and mount behaviors by urinary cues from female mice (SJL/J) was assessed in two experiments. Experiment 1 examined the efficacy of urine from ovariectomized and intact females in modulating the behavior of sexually experienced and inexperienced males vis-à-vis the control substance, water. It was found that urine from ovariectomized females showed no behavior-modulating properties, whereas urine from intact females decreased attack but increased sniff and mount behaviors in male mice. These findings are indicative of ovarian-dependent chemosignals contained in the urine of only intact females. Prior sexual experience had no effect on behavior of males when exposed to urine from ovariectomized females. Sniff and mount behaviors were, however, increased when sexually experienced males were exposed to urine from intact females. Using sexually inexperienced males, Experiment 2 assessed the chemosignal activity of urine from females in each of four estrous phases. It was determined that urine from proestrous and estrous females increased sniff and mount behaviors and decreased attack behavior. Urine from metaestrous females was shown to possess only sniff-inducing and mount-reducing chemosignal properties. Urine from females in diestrous lacked attack- and mount-modulating chemocues but may possess a sniff-inducing chemosignal.