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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Live-cell Imaging of Platelet Degranulation and Secretion Under Flow
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Evaluating stored platelet shape change using imaging flow cytometry.

Tahsin Özpolat1, Olga Yakovenko1, Anastasiia Stratiievska1

  • 1Department's name is Research Institute, Bloodworks Northwest Research Institute, Seattle, WA, USA.

Platelets
|November 3, 2022
PubMed
Summary
This summary is machine-generated.

A new imaging flow cytometry method offers an unbiased assessment of platelet shape change during storage. This tool aids in evaluating platelet quality and developing strategies to mitigate cold-induced storage lesions.

Keywords:
Cold storageKunicki morphology scoreimaging flow cytometryplateletsshape changetransfusion

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Area of Science:

  • Hematology
  • Biotechnology
  • Cell Biology

Background:

  • Platelet storage quality is traditionally assessed by subjective morphology scoring.
  • Cold storage of platelets is being reconsidered for benefits like reduced bacterial growth.
  • Cold exposure induces significant platelet shape changes, impacting traditional assessment methods.

Purpose of the Study:

  • To develop a simpler, unbiased screening tool for temperature-induced platelet shape change.
  • To quantify platelet shape changes using imaging flow cytometry.
  • To aid in screening therapeutic measures for cold storage lesions.

Main Methods:

  • Developed an imaging flow cytometry assay to measure platelet shape change.
  • Defined platelet shape based on the ratio of transverse to longitudinal axes in 2D.
  • Analyzed temperature and time-dependent shape changes in mouse and human platelets.

Main Results:

  • The assay accurately detects temperature-induced platelet shape changes from fusiform to circular.
  • Shape change is dependent on both temperature and storage duration.
  • Results correlate with known 3D shape changes from discoid to spherical.

Conclusions:

  • The described imaging flow cytometry method is a valuable tool for assessing platelet shape change.
  • This assay can detect differences in response to agonists and temperature.
  • The method will assist in screening therapies to mitigate cold-induced storage lesions.