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A Lanthanide Upconversion Nanothermometer for Precise Temperature Mapping on Immune Cell Membrane.

Hanyu Liang1,2, Kaidong Yang1, Yating Yang1

  • 1MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.

Nano Letters
|November 3, 2022
PubMed
Summary

This study introduces a novel nanothermometer for precise cell membrane temperature mapping. This method enables efficient immune cell activation by identifying optimal temperature conditions for calcium influx in T cells.

Keywords:
cell imaginglanthanidenanoprobethermometryupconversion luminescence

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Precise monitoring of intracellular temperature is crucial for understanding cellular functions.
  • Existing methods face challenges in accurately probing localized temperature within living cells.

Purpose of the Study:

  • To develop a novel method for in situ temperature mapping on immune cell membranes.
  • To investigate the relationship between temperature and calcium influx in T cells for enhanced immune cell activation.

Main Methods:

  • Utilized lanthanide-doped upconversion nanoparticles as a nanothermometer.
  • Employed metabolic labeling and click chemistry for cell membrane targeting.
  • Developed a custom upconversion hyperspectral microscope for simultaneous temperature and calcium imaging.

Main Results:

  • Achieved sensitive in situ temperature monitoring on T cell membranes with a sensitivity of 1.4% K⁻¹.
  • Successfully mapped temperature changes and visualized intracellular calcium influx.
  • Identified specific temperature conditions that facilitate thermally stimulated calcium influx, leading to high-efficiency immune cell activation.

Conclusions:

  • This nanothermometer strategy offers a new tool for real-time temperature monitoring in living cells.
  • Findings advance the understanding of thermally dependent biological processes and immune cell regulation.
  • Enables precise control over cellular functions through temperature modulation.