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Updated: Aug 23, 2025

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Endothelial cell activation by interleukin-1 and extracellular matrix laminin-10 occurs via the YAP signalling
Hannah Thurgur1, Jeffrey Penny2, Emmanuel Pinteaux1
1Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance NHS Group, Manchester, United Kingdom; Division of Neuroscience, Faculty of Biology, Medicine and Health; A.V. Hill Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom.
Abstract:
Laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) repair after hypoxia and inflammation. Here we investigated the signalling mechanisms regulated by LM-10 in human brain endothelial cell line hCMEC/D3 in response to interleukin(IL)-1beta(β) in vitro. LM-10 promoted endothelial proliferation and repair of an endothelial monolayer after scratch injury, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression. IL-1β and LM-10 regulated YAP signalling pathway in endothelial cells leading to differential expression of YAP target genes, ctgf and serpine-1, providing evidence that the YAP signalling pathway could be a new therapeutic target for the treatment of BBB dysfunction in CNS diseases.
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