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Real-Time Whole Genome Sequencing to Guide Patient-Tailored Therapy of Severe Acute Respiratory Syndrome Coronavirus
Luke B Snell1, Adela Alcolea-Medina1, Themoula Charalampous1
1Department of Infection, Guy's and St. Thomas' NHS Foundation Trust, London, UK.
Abstract:
The management of coronavirus disease 2019 has become more complex due to the expansion of available therapies. The presence of severe acute respiratory syndrome coronavirus 2 variants and mutations further complicates treatment due to their differing susceptibilities to therapies. Here we outline the use of real-time whole genome sequencing to detect persistent infection, evaluate for mutations confering resistance to treatments, and guide treatment decisions.
Insights
Real-time whole genome sequencing helps manage coronavirus disease 2019 by detecting persistent infections and identifying mutations. This approach guides effective treatment decisions against SARS-CoV-2 variants.
Area of Science:
- Virology
- Genomics
- Infectious Diseases
Background:
- Coronavirus disease 2019 (COVID-19) management is complicated by numerous therapies.
- Emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants and mutations impact treatment efficacy.
Purpose of the Study:
- To outline the application of real-time whole genome sequencing in COVID-19 management.
- To demonstrate how sequencing can guide personalized treatment strategies.
Main Methods:
- Utilizing real-time whole genome sequencing.
- Detecting persistent SARS-CoV-2 infections.
- Identifying mutations associated with therapeutic resistance.
Main Results:
- Real-time sequencing enables timely detection of persistent infections.
- The method identifies specific mutations conferring resistance to antiviral therapies.
- Genomic data informs clinical decisions for COVID-19 patients.
Conclusions:
- Real-time whole genome sequencing is a valuable tool for complex COVID-19 cases.
- This approach enhances the precision of treatment selection for SARS-CoV-2 infections.
- Genomic surveillance aids in overcoming challenges posed by viral evolution in treatment.
