Carbon dots derived from peptone as "off-on" fluorescent probes for the detection of oxalic acid

A simple and rapid microwave heating approach was reported for the preparation of water soluble carbon dots (CDs) using peptone as carbon source with the assistance of ethylenediamine. Several characterization techniques such as transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) were employed to analyze CDs. The optical properties of synthesized CDs were examined by UV-vis and fluorescence spectroscopy. The CDs exhibit strong blue emission under 365 nm UV lamp and have the excitation and pH (2.0-12.0) dependent emission behavior. The fluorescence intensity of CDs can be selectively quenched by Co²⁺ via dynamic mechanism, while the addition of oxalic acid (OA) results in a remarkable recovery of the fluorescence intensity due to the strong coordination binding between oxalic acid and Co²⁺. Hence, the prepared CDs can conveniently serve as "off-on" fluorescent probes for highly sensitive determination of oxalic acid. The wide linear range is 0.5-70 mg/L with a low detection limit of 0.288 mg/L. Furthermore, the probes were successfully applied to detect oxalic acid in tomato and cherry tomato samples with the recovery of 96.4 %-106.4 % and the relative standard deviation lower than 0.25 %.