Colorimetric aptasensor for fumonisin B₁ detection based on the DNA tetrahedra-functionalized magnetic beads and DNA...

The toxicity and incidence of fumonisin B₁ (FB₁) pose a major challenge to public health and the environment, prompting the development of alternative quantitative strategies for FB₁. Herein, a colorimetric aptasensor was constructed based on DNA tetrahedra-functionalized magnetic beads (MBs) and DNA hydrogel-coated Mn-Zr bimetallic metal-organic frameworks-based nanozyme (MOFzyme). Initially, MBs functionalized by DNA tetrahedra demonstrated excellent capturing capability for FB₁. Along with the capture of FB₁, catalyst DNA (C) was released into the supernatant. Aided by fuel DNA (F), C can trigger continuous cleavage of the main chains and cross-linking points of the DNA hydrogel through an entropy-driven DNA circuit integrated into the hydrogel coating. Subsequently, the bimetallic MOFzyme encapsulated inside the DNA hydrogel was exposed and exerted its superb peroxidase-like activity, producing a colorimetric signal whose intensity was positively dependent on the amount of FB₁. The developed aptasensor exhibited good linearity in the range of 5 × 10^-4 to 50 ng mL^-1 with a limit of detection (LOD) of 0.38 pg mL^-1, and reasonable specificity in different matrices. Furthermore, the aptasensor was successfully applied to quantify FB₁ in actual samples with recoveries fell within 92.25 %- 108.00 %, showing its great potential in environmental monitoring and food safety.