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Polydatin protects against gouty nephropathy by inhibiting renal tubular cell pyroptosis

Xingliang Shi1,2, Lili Zhuang1,2, Zeqing Zhai1,2

  • 1Department of Rheumatology and Immunology, The Third Affiliated Hospital, Southern Medical University, Guangzhou, China.

International Journal of Rheumatic Diseases
|November 3, 2022
PubMed
Abstract

Insights

Polydatin (PD) protects against gouty nephropathy (GN) in mice by inhibiting pyroptosis. This involves downregulating NLRP3, caspase-1, and gasdermin D (GSDMD) proteins, offering a potential therapeutic strategy for GN.

Area of Science:

  • Nephrology
  • Pharmacology
  • Cell Biology

Background:

  • Gouty nephropathy (GN) is a serious complication of gout, characterized by kidney damage.
  • Pyroptosis, a pro-inflammatory form of programmed cell death, plays a significant role in the pathogenesis of GN.
  • Identifying therapeutic agents that can mitigate GN progression by targeting pyroptosis is crucial.

Purpose of the Study:

  • To investigate the protective effects of polydatin (PD) against gouty nephropathy (GN) in a mouse model.
  • To elucidate the underlying mechanism of PD's action, focusing on its impact on pyroptosis and related proteins.

Main Methods:

  • A mouse model of GN was established using hypoxanthine and potassium oxonate.
  • Mice were treated with polydatin (PD) and compared to control and GN-only groups.
  • Kidney histology, serum markers (uric acid, BUN, creatinine), and protein expression (NLRP3, caspase-1, GSDMD) were analyzed.

Main Results:

  • Polydatin (PD) treatment significantly ameliorated kidney tissue damage in GN mice.
  • PD reversed abnormal levels of serum uric acid and blood urea nitrogen.
  • PD inhibited the expression of NLRP3, caspase-1, and GSDMD, thereby reducing renal tubular epithelial cell pyroptosis.

Conclusions:

  • Polydatin (PD) demonstrates a significant protective effect against gouty nephropathy (GN) in mice.
  • The protective mechanism involves the downregulation of NLRP3, caspase-1, and GSDMD proteins.
  • PD's inhibition of pyroptosis suggests its potential as a therapeutic agent for GN.
Keywords:
NOD-like receptor protein 3gasdermin Dgouty nephropathypolydatinpyroptosis

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