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Related Experiment Video

Updated: Aug 23, 2025

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
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High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCR.

Yuanyuan Zeng1, Lili Zhou2, Zhongzhu Yang1

  • 1State Key Laboratory of Southwestern Chinese Medicine Resources, College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 611137, China.

Analytica Chimica Acta
|November 3, 2022
PubMed
Summary

This study introduces a graphene oxide-multiplex qPCR method for sensitive SARS-CoV-2 detection. The new assay significantly improves detection limits for early and accurate diagnosis of the virus.

Keywords:
DetectionGraphene oxideHigh sensitivityMultiplex qPCRSARS-CoV-2

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Area of Science:

  • Molecular Biology
  • Nanotechnology
  • Virology

Background:

  • Accurate diagnosis of SARS-CoV-2 is crucial for pandemic control.
  • Conventional RT-qPCR may fail to detect low viral loads, impacting early diagnosis.
  • Graphene oxide (GO) shows potential for enhancing molecular detection methods due to its ssDNA adsorption properties.

Purpose of the Study:

  • To develop a highly sensitive method for simultaneous detection of SARS-CoV-2 genes.
  • To improve the limit of detection for SARS-CoV-2 compared to standard techniques.
  • To evaluate the efficacy of a novel graphene oxide-multiplex qPCR assay.

Main Methods:

  • A GO-multiplex qPCR system was designed for simultaneous detection of two SARS-CoV-2 genes (RdRP and E) and a reference gene (RNase P).
  • Graphene oxide pre-absorbs forward primers to form specific composites, confining target amplification.
  • The assay's sensitivity was compared against conventional multiplex qPCR.

Main Results:

  • The GO-multiplex qPCR assay demonstrated a 10-fold reduction in the limit of detection.
  • The assay achieved a limit of detection as low as 10 copies/reaction.
  • Simultaneous detection of multiple genes was successfully achieved with enhanced sensitivity.

Conclusions:

  • The GO-multiplex qPCR assay offers significantly improved sensitivity for SARS-CoV-2 detection.
  • This method can effectively identify low viral loads, aiding in early and accurate diagnosis.
  • Graphene oxide integration presents a promising strategy for enhancing molecular diagnostic tools for infectious diseases.