Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compartmentalizing the S period.

M H Navarrete, C de la Torre, J B Schvartzman

    Cell Biology International Reports
    |November 1, 1978
    PubMed
    Summary

    This study introduces a novel method for interphase analysis, enhancing resolution for tissues unsuitable for flow cytometry. The technique confirms hydroxyurea

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cardiomyocyte-specific RXFP1 overexpression protects against pressure overload-induced cardiac dysfunction independently of relaxin.

    Biochemical pharmacology·2024
    Same author

    Combined approach for paediatric recurrent antrochoanal polyp: a single-centre case series of 27 children.

    The Journal of laryngology and otology·2019
    Same author

    [Pulmonary lobectomy in children: the sooner the better?]

    Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica·2018
    Same author

    Practice Models in Teledermatology in Spain: Longitudinal Study, 2009-2014.

    Actas dermo-sifiliograficas·2018
    Same author

    Drug Delivery Nanosystems for the Localized Treatment of Glioblastoma Multiforme.

    Materials (Basel, Switzerland)·2018
    Same author

    [Predictive factors of gangrenous post-appendectomy intra-abdominal abscess. A case-control study].

    Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica·2018

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Genetics

    Background:

    • Traditional cytofluorometric techniques are not applicable to all tissue types for interphase analysis.
    • Accurate cell cycle phase estimation is crucial for understanding tissue growth and response to treatments.

    Purpose of the Study:

    • To develop a high-resolution method for interphase analysis in tissues where cell flow cytometry is not feasible.
    • To provide an alternative approach for detailed cell cycle phase determination.

    Main Methods:

    • Combines 3H-thymidine pulse labeling for cell cycle phase estimation (G1, S, G2, mitosis).
    • Utilizes cytophotometry on Feulgen-stained nuclei to group cells by DNA content.
    • Superimposes labeling and DNA content data to define three distinct compartments within the S phase.

    Main Results:

    • The method successfully resolved different stages within the S phase.
    • Demonstrated increased resolution in interphase analysis compared to existing methods.
    • Confirmed that hydroxyurea treatment accumulates cycling cells in early S phase of Allium cepa L. root meristems after one cell cycle time.

    Conclusions:

    • The developed method offers a valuable alternative for high-resolution interphase analysis in various tissue types.
    • This technique provides deeper insights into cell cycle dynamics and drug effects.
    • The findings validate the method's precision in identifying cell cycle perturbations.

    Related Experiment Videos