A Manganese-Based Metal-Organic Framework as a Cold-Adapted Nanozyme

Area of Science:

• Biomimetic chemistry
• Nanotechnology
• Enzyme engineering

Background:

• Cold-adapted enzymes are crucial for catalytic medicine at low temperatures.
• Existing enzymes often have limited activity or stability in cryogenic conditions.
• Developing artificial enzymes with broad temperature tolerance is a significant challenge.

Purpose of the Study:

• To design and synthesize a novel cold-adapted nanozyme.
• To investigate its catalytic activity and stability across various temperatures.
• To explore its application in low-temperature antiviral strategies.

Main Methods:

• Synthesis of a manganese-based nanosized metal-organic framework (nMnBTC) as an oxidase mimetic.
• Evaluation of nMnBTC activity at temperatures ranging from 0°C to 45°C.
• Testing the efficacy of nMnBTC in inactivating influenza virus H1N1 at -20°C.

Main Results:

• The synthesized nMnBTC exhibited excellent oxidase-like activity at 0°C.
• nMnBTC demonstrated remarkable stability with minimal activity loss up to 45°C.
• The nanozyme successfully inactivated influenza virus H1N1 at -20°C, showcasing its antiviral potential.

Conclusions:

• nMnBTC represents a novel, highly efficient, and stable cold-adapted nanozyme.
• Its broad temperature activity challenges traditional enzyme performance expectations.
• This work offers a new avenue for developing artificial enzymes and biomedical applications in cryogenic environments.