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Related Experiment Video

Updated: Aug 23, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

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Microfluidic Paper-based Device for Medicinal Diagnosis.

Atchara Lomae1,2, Pattarachaya Preechakasedkit2, Kanyapat Teekayupak1

  • 1Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.

Current Topics in Medicinal Chemistry
|November 4, 2022
PubMed
Summary

Microfluidic paper-based analytical devices (μPADs) offer a low-cost solution for point-of-care testing, addressing challenges in cost and resources. This review details their fabrication, design, and diagnostic applications.

Keywords:
Device designLateral flow assay (LFA)Medicinal diagnosisMicrofluidic paper-based analytical devices (μPADs)Paper-based analytical devicePointof- care testing (POC)Three-dimensional (3D) configuration.Two-dimensional (2D) configurations

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Last Updated: Aug 23, 2025

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Point-of-care testing (POCT) demand is rising due to immediate results and ease of use for home testing.
  • Traditional POCT faces challenges with high costs and limited resources.
  • Microfluidic paper-based analytical devices (μPADs) offer a cost-effective alternative using low-cost paper substrates.

Purpose of the Study:

  • To review fabrication methods and device designs (2D/3D) of μPADs.
  • To analyze the advantages and disadvantages of different μPAD configurations.
  • To focus on updated μPAD applications for motif identification in medicinal diagnostics.

Main Methods:

  • Described the evolution from dipstick and lateral flow assays (LFA) to μPADs.
  • Compared patterned structure fabrication techniques, including equipment, benefits, and drawbacks.
  • Introduced 2D and 3D microfluidic device designs and modifications.

Main Results:

  • Detailed various μPAD designs integrated with detection methods: colorimetry, electrochemistry, fluorescence, chemiluminescence, electrochemiluminescence, and SER-based sensors.
  • Highlighted the simple fabrication, low cost, capillary-driven flow, reagent storage, and multistep assay capabilities of μPADs.
  • Showcased updated prototypes representing real-life application breakthroughs.

Conclusions:

  • Discussed the commercialization potential of μPADs in medicinal diagnostics.
  • Emphasized the state-of-the-art advancements in μPADs for medical diagnosis.
  • Confirmed μPADs as a promising tool for accessible and affordable healthcare solutions.