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Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

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Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
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Related Experiment Video

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Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
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Monkeypox virus diagnosis and laboratory testing.

Mohsen Nakhaie1, Nasir Arefinia2, Javad Charostad3

  • 1Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.

Reviews in Medical Virology
|November 4, 2022
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Rapid detection of monkeypox virus (MPXV) is crucial for global health. This review details MPXV detection methods, biosafety, and sample handling to combat the rising threat.

Keywords:
diagnosislaboratory testingmonkeypox virus

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Area of Science:

  • Virology
  • Infectious Diseases
  • Public Health

Background:

  • The global spread of monkeypox virus (MPXV) presents a significant public health challenge, particularly during the ongoing COVID-19 pandemic.
  • The re-emergence and increasing incidence in non-endemic regions underscore the urgent need for effective control measures.

Purpose of the Study:

  • To review and summarize applicable strategies for the primary detection and confirmation of MPXV.
  • To highlight critical aspects of MPXV diagnosis, including biosafety, sample management, and waste disposal.
  • To evaluate the advantages and disadvantages of various diagnostic assays for MPXV.

Main Methods:

  • Review of current literature on MPXV detection methodologies.
  • Analysis of diagnostic techniques including molecular assays, immunoassays, histopathology, electron microscopy, genomic sequencing, and cell culture.
  • Examination of essential procedures for specimen collection, transportation, storage, and biosafety protocols.

Main Results:

  • Various diagnostic assays for MPXV detection have been illustrated, each with specific strengths and limitations.
  • Key considerations for biosafety, laboratory requirements, and standard operating procedures for handling MPXV are discussed.
  • Information on specimen collection, transportation, storage, and waste disposal is provided to ensure safe and accurate diagnostics.

Conclusions:

  • Accurate and rapid identification of MPXV is vital for assessing the global extent of the outbreak and implementing preventive strategies.
  • Understanding the nuances of different diagnostic approaches is essential for effective MPXV surveillance and control.
  • Comprehensive guidelines on sample handling and biosafety are critical for managing MPXV outbreaks effectively.