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Updated: Aug 23, 2025
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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Genetic tools to dissect functions of long noncoding RNAs
Anna Nawrocka1, Vedrana Andric1, Alena Shkumatava1
1Institut Curie, PSL Research University, CNRS UMR3215, INSERM U934, Paris, France.
IUBMB Life
|November 4, 2022
Summary
Genetic inactivation of long noncoding RNAs (lncRNAs) is crucial for understanding their regulatory roles. This review explores genome editing and comparative genomics strategies to overcome challenges in targeting these abundant noncoding transcripts.
Area of Science:
- Molecular Biology
- Genetics
- Genomics
Background:
- Long noncoding RNAs (lncRNAs) are abundant noncoding transcripts with significant regulatory functions.
- Understanding lncRNA biological roles necessitates reliable genetic inactivation strategies.
- lncRNA targeting presents unique challenges due to large genomic loci, transcript stability, and rapid sequence evolution.
Approach:
- Review of genome editing techniques for generating lncRNA mutant alleles in model organisms.
- Discussion of challenges in lncRNA genetic targeting, including large loci and sequence variability.
- Highlighting comparative genomics for identifying conserved functional lncRNA sequence motifs.
Key Points:
- Genome editing advances offer new tools for dissecting lncRNA functions.
- lncRNA inactivation strategies must account for their distinct genomic and sequence characteristics.
- Comparative genomics aids in pinpointing critical functional elements within lncRNAs.
Conclusions:
- Developing effective genetic strategies is essential for functional lncRNA research.
- Combined genome editing and comparative genomics approaches enhance the ability to study lncRNA roles.
- Targeting conserved lncRNA motifs offers a promising avenue for functional abrogation.
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