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Updated: Aug 23, 2025
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Isolation of Murine Bone Marrow-Derived Neutrophils for Infection Modeling
Laurel Duty1, Amber M Paul2,3,4,5
1Department of Cell and Molecular Biology, University of Southern Mississippi, Hattiesburg, MS, USA.
Abstract:
West Nile virus (WNV) is a single-stranded-RNA flavivirus that can cause neurological illnesses. The ability of WNV to cause neurological illnesses is dependent on the virus' ability to gain access into the brain. However, the mechanisms by which WNV enters the brain are elusive, with evidence that neutrophils act as vehicles for viral delivery. To determine the role of neutrophils in WNV central nervous system delivery, modified protocols for the isolation and migration of neutrophils from mouse bone marrow for in vitro WNV infection modeling is described.
