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Differential Expression of Genes Related to Innate Immune Responses in Ex Vivo Spinal Cord and Cerebellar Slice Cultures Infected with West Nile Virus.

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Related Experiment Video

Updated: Aug 23, 2025

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Published on: December 28, 2017

Protocol to Study West Nile Virus Infection in Brain Slices In Vitro.

Parminder J S Vig¹

¹Department of Neurology, University of Mississippi Medical Center, Jackson, MS, USA. pvig@umc.edu.

Methods in Molecular Biology (Clifton, N.J.)

|November 4, 2022

Summary

This summary is machine-generated.

Ex vivo brain slice cultures preserve central nervous system (CNS) cytoarchitecture, enabling detailed study of neural and glial cell connectivity. This method allows investigation of West Nile virus effects on cerebellar cells in a near-native environment.

Keywords:

Calcium-binding proteins Cerebellum Glia Purkinje cells Slice cultures West Nile

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Last Updated: Aug 23, 2025

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

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In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

Published on: August 18, 2020

Area of Science:

Neuroscience
Virology
Cell Biology

Background:

Ex vivo brain slice cultures maintain central nervous system (CNS) cytoarchitecture, closely mimicking the in vivo environment.
These cultures facilitate the study of complex neuronal and glial connectivity, which is not achievable with standard in vitro cell lines.
Understanding cellular interactions under normal and pathological conditions is crucial for neurological research.

Purpose of the Study:

To describe a method for preparing ex vivo cerebellar slice cultures from mice.
To establish a protocol for investigating the impact of West Nile virus infection on cerebellar cells within these slice cultures.
To provide a model for studying viral pathogenesis in a complex neural network.

Main Methods:

Preparation of ex vivo mouse cerebellar slice cultures.
Infection of slice cultures with West Nile virus.
Microscopic and potentially molecular analysis of infected cerebellar cells.

Main Results:

Successful establishment and maintenance of viable mouse cerebellar slice cultures.
Demonstration of West Nile virus infectivity and potential cytopathic effects within the cerebellar slice model.
Observation of specific cellular responses to West Nile virus infection in the cerebellum.

Conclusions:

Ex vivo cerebellar slice cultures offer a valuable platform for studying viral infections of the central nervous system.
This model system allows for detailed analysis of viral effects on neuronal and glial networks.
The described protocol facilitates research into West Nile virus pathogenesis and potential therapeutic interventions.