Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

95
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
95
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

142
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
142

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nasopharyngeal adenoid cystic carcinoma: magnetic resonance imaging features in ten cases.

Chinese journal of cancer·2011
Same author

Amyloid fibril formation by the glaucoma-associated olfactomedin domain of myocilin.

Journal of molecular biology·2011
Same author

Moxibustion down-regulates colonic epithelial cell apoptosis and repairs tight junctions in rats with Crohn's disease.

World journal of gastroenterology·2011
Same author

[Impact of metoprolol use in the treatment of patients with electrical-storm after cardioverter defibrillators implantation].

Zhonghua xin xue guan bing za zhi·2011
Same author

Effect of intravitreal cell-based produced glucagon-like peptide-1 on Bcl and BAX expression in the optic nerve crush model.

Acta ophthalmologica·2011
Same author

Meta-analysis of genome-wide association studies identifies eight new loci for type 2 diabetes in east Asians.

Nature genetics·2011
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 23, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K

Studying Virus-Host Interactions with CRISPR Technology.

Rong Zhang1

  • 1Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China. rong_zhang@fudan.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|November 4, 2022
PubMed
Summary
This summary is machine-generated.

West Nile virus (WNV) infection requires specific host factors. This study outlines CRISPR knockout screening methods to identify these essential factors, paving the way for new antiviral strategies against WNV and other viruses.

Keywords:
CRISPRGenome-wide screenHost factorVirus-host interactionsWest Nile virus

More Related Videos

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.4K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K

Related Experiment Videos

Last Updated: Aug 23, 2025

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
11:28

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Published on: May 23, 2016

17.7K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.4K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K

Area of Science:

  • Virology
  • Genetics
  • Infectious Diseases

Background:

  • West Nile virus (WNV) is a significant public health concern due to the lack of vaccines or specific antiviral treatments.
  • Understanding virus-host interactions is crucial for elucidating WNV pathogenesis and transmission.
  • Identifying host factors essential for WNV infection can reveal potential antiviral targets.

Purpose of the Study:

  • To describe the methodology for performing a CRISPR knockout screen to identify host factors critical for WNV infection.
  • To provide a framework for applying CRISPR screening to discover host factors for other viral infections.

Main Methods:

  • Utilizing CRISPR-Cas9 gene-editing technology for genome-wide screening.
  • Perturbating host gene expression to identify genes essential for WNV replication.
  • Performing unbiased screens to discover novel host-dependency factors.

Main Results:

  • The described CRISPR knockout screen methodology enables the identification of host factors crucial for WNV infection.
  • This approach is adaptable for discovering host factors essential for a wide range of viral pathogens.
  • The study provides a foundation for future research into WNV-host interactions and therapeutic targets.

Conclusions:

  • CRISPR knockout screens are a powerful tool for identifying host factors essential for viral infections like WNV.
  • This methodology facilitates the discovery of novel antiviral targets and enhances our understanding of virus-host biology.
  • The described approach has broad applicability for studying other viral diseases and developing targeted interventions.