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Biocompatibility and biological activity of C70 fullerene adduct with L-threonine (C70(C4H9NO3)2)
Vladimir V Sharoyko1, Evgenii B Serebriakov2, Sergei V Ageev3
1Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo Str., Saint Petersburg, 197022, Russia; Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Pr., Saint Petersburg, 198504, Russia; A. M. Granov Russian Research Centre for Radiology and Surgical Technologies, 70 Leningradskaia Str., Saint Petersburg, 197758, Russia.
Abstract:
The aim of this work is to synthesise and study the biocompatibility and biological activity of the C70 fullerene adduct with l-threonine (C70-Thr). The obtained adduct was identified using a complex of physicochemical methods, namely, 13C NMR spectroscopy, IR spectroscopy, thermogravimetric analysis, electron spectroscopy, elemental analysis, and high-performance liquid chromatography. The study of biocompatibility and biological activity of the C70-Thr adduct included the study of haemocompatibility (haemolysis, platelet aggregation, plasma coagulation haemostasis, binding to human serum albumin, esterase activity), antiradical activity, cytotoxicity, cell proliferation, and interaction with DNA (determination of the DNA binding constant and genotoxicity).
