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Updated: Aug 23, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Bioaugmentation reconstructed nitrogen metabolism in full-scale simultaneous partial nitrification-denitrification,
Huimin Liu1, Dejin Liu1, Zhenyu Huang1
1School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou, Guangdong 510006, PR China.
Abstract:
To enhance nitrogen removal of fermentation pharmaceutical wastewater with high nitrogen load, a full-scale process based on simultaneous partial nitrification-denitrification/ anammox/ sulfur autotrophic denitrification (SPAS) was established via inoculating with bioaugmentation consortia in a modified two-stage AO. More than 93 % TN and 98 % NH4+-N removal were obtained at a rate of 0.8 kg-N/ m3/d in the first A/O stage, in which short-cut SND was involved with 96.05 % ESND when bioaugmented with SND, while S0-SAD could coordinate with anammox to exert further deep denitrification in the second A/O stage. KEGG analysis demonstrated that the SPAS process was synergism of HD, PN/PDN, SND, SAD and anammox metabolism, bioaugmentation could significantly up-regulate genes related to microbial metabolism (TCA cycle, Carbon metabolism, ABC transporters) and environmental adaptation (Two-component system, Quorum sensing) based on the FAPROTAX and Picrust2 functional prediction. This study provided a new perspective in engineering applications.
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