Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.8K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.8K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

24.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell transcriptomics reveals differences between chorionic and basal plate cytotrophoblasts and 2D-cultured trophoblast stem cells.

Communications biology·2026
Same author

Clinicogenomic Real-World Data Enable Prediction of Hospital Readmissions at a Comprehensive Cancer Center.

JCO clinical cancer informatics·2026
Same author

Derivation of functional early gestation decidual natural killer cell subtypes from induced pluripotent stem cells.

Stem cells (Dayton, Ohio)·2026
Same author

The association between health-related quality of life and the meaning of life in community-dwelling older adults.

BMC geriatrics·2026
Same author

FAK inhibition in ovarian cancer releases omega-3 fatty acids to program CXCL13-producing anti-tumor resident peritoneal macrophages.

Cell reports·2026
Same author

Determinants of Placental Versus Maternal Preeclampsia.

Hypertension (Dallas, Tex. : 1979)·2026

Related Experiment Video

Updated: Aug 23, 2025

Author Spotlight: Enhancing PSC-to-Functional Cell Differentiation Using ML Models Based on Live-Cell Bright-Field Imaging
11:38

Author Spotlight: Enhancing PSC-to-Functional Cell Differentiation Using ML Models Based on Live-Cell Bright-Field Imaging

Published on: October 4, 2024

666

Modeling placental development and disease using human pluripotent stem cells.

Robert Morey1, Tony Bui1, Kathleen M Fisch2

  • 1Department of Pathology, University of California San Diego, La Jolla, CA, 92093, USA; Sanford Consortium for Regenerative Medicine, University of California San Diego, La Jolla, CA, 92093, USA.

Placenta
|November 4, 2022
PubMed
Summary

New in vitro models of human trophoblast stem cells (hTSC) and trophoblast organoids improve the study of early placental development and pregnancy diseases. These models overcome limitations of previous cell lines for better research insights.

Keywords:
Disease modelingHuman pluripotent stem cellsTrophoblast organoidTrophoblast stem cells

More Related Videos

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
08:21

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

8.7K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

7.9K

Related Experiment Videos

Last Updated: Aug 23, 2025

Author Spotlight: Enhancing PSC-to-Functional Cell Differentiation Using ML Models Based on Live-Cell Bright-Field Imaging
11:38

Author Spotlight: Enhancing PSC-to-Functional Cell Differentiation Using ML Models Based on Live-Cell Bright-Field Imaging

Published on: October 4, 2024

666
In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
08:21

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Published on: March 16, 2017

8.7K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

7.9K

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Traditional models for studying human trophoblast, such as mouse stem cells and immortalized cell lines, present significant discrepancies with in vivo human trophoblast.
  • Difficulties in culturing primary trophoblast and establishing human trophoblast stem cell (hTSC) lines have historically limited research.
  • Human pluripotent stem cells (hPSCs) have shown potential for trophoblast differentiation, offering a promising alternative model.

Purpose of the Study:

  • To present advanced in vitro models for human trophoblast differentiation, including primary and hPSC-derived TSC and trophoblast organoids.
  • To provide tools for studying early placental development and its associated diseases.
  • To highlight recent technological advancements that have expanded the capacity to understand trophoblast biology.

Main Methods:

  • Derivation of hTSC from pre-implantation blastocysts or early gestation placenta.
  • Generation of trophoblast organoids from early gestation placenta.
  • Maintenance of TSC as both monolayers and 3-dimensional organoids.
  • Application of established differentiation protocols to hPSCs for trophoblast generation.

Main Results:

  • Development of robust methods for deriving and maintaining hTSC and trophoblast organoids.
  • Successful application of these methods to hPSC-derived TSC and organoids.
  • Significant expansion in the field's ability to study trophoblast differentiation and disease.

Conclusions:

  • In vitro models, including primary and hPSC-derived TSC and trophoblast organoids, are crucial tools for advancing the study of early human placental development.
  • These models offer improved insights into trophoblast differentiation and their role in pregnancy-related diseases.
  • Technological advancements have revolutionized the study of trophoblast, enabling deeper understanding of placental function and pathology.