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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Aug 23, 2025

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Hyperbolic material enhanced scattering nanoscopy for label-free super-resolution imaging.

Yeon Ui Lee^1,2, Shilong Li^1,3, G Bimananda M Wisna⁴

¹Department of Electrical and Computer Engineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.

Nature Communications

|November 5, 2022

Summary

Hyperbolic material enhanced scattering (HMES) nanoscopy achieves label-free super-resolution imaging by tailoring light-matter interactions. This method breaks the diffraction limit, offering a 5.5-fold resolution improvement for applications where fluorescence is difficult.

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Last Updated: Aug 23, 2025

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

Area of Science:

Optics
Nanotechnology
Materials Science

Background:

Fluorescence super-resolution microscopy has advanced nanoscale imaging.
Label-free super-resolution techniques show limited improvement over the diffraction limit.

Purpose of the Study:

To demonstrate a novel label-free super-resolution imaging method using hyperbolic materials.
To overcome the limitations of existing label-free super-resolution technologies.

Main Methods:

Developed hyperbolic material enhanced scattering (HMES) nanoscopy.
Utilized light-matter interaction between specimens and a hyperbolic material substrate.
Excited confined evanescent hyperbolic polariton modes with dark-field detection.

Main Results:

Achieved high-contrast scattering images with a spatial resolution of approximately 80 nm.
Demonstrated a 5.5-fold resolution improvement beyond the diffraction limit at 532 nm wavelength and 0.6 NA.
Showcased the efficacy of HMES nanoscopy for label-free super-resolution imaging.

Conclusions:

HMES nanoscopy offers a viable label-free approach for super-resolution imaging.
This technique is particularly valuable when fluorescence imaging is not feasible.
HMES nanoscopy significantly enhances optical resolution for nanoscale investigations.