A NIR Fluorescent Probe Benzopyrylium Perchlorate-based for Visual Sensing and Imaging of SO₂ Derivatives in Living...

Endogenous sulfur dioxide (SO₂), as a gas signal molecule, has a certain physiological functions. Understanding the role of endogenous SO₂ in human physiology and pathology is of great significance to the biological characteristics of SO₂,which bring challenges to develop fluorescent probes with excellent performance. Herein, we rationally designed and constructed a novel near-infrared bioprobe benzaldehyde-benzopyrylium (BBp) by employing the nucleophilic addition benzopyrylium perchlorate fluorophore and benzaldehyde moiety by means of C = C/C = O group that serves as both fluorescence reporting unit. Probe BBp exhibit excellent sensing performance with fluorescent "On - Off"rapid response (100 s) and long-wavelength emission (670 nm). With the treatment of HSO₃^-, the color of BBp solution obviously varies from purple to colorless, and the fluorescent color varies from red to colorless. By the fluorescence and colorimetric changes, probe BBp was capable of sensitive determination HSO₃^- with low limits of detection (LOD) of 0.43 μM, realizing visual quantitative monitoring SO₂ derivative levels. Due to the low phototoxicity and good biocompatibility, it was successfully applied to monitor SO₂ derivatives and fluorescence imaging in HepG2 and HeLa living cells. Hopefully, this work supplies a new strategy for designing NIR fluorescent probes for quantitative determination SO₂ derivatives in biological samples.