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A rapid ELISA for the detection of matrix metallopeptidase 9 using a recombinant Fab-type antibody

Hui-Seon Yun1, Jong-Pyo Kim1, Eun-Jung Kim2

  • 1Department of Biological and Chemical Engineering, Hongik University, Sejong, 30016, Republic of Korea.

Biochemical and Biophysical Research Communications
|November 5, 2022
PubMed

Insights

Researchers developed a rapid, high-purity recombinant antibody for Matrix metalloproteinase 9 (MMP9) detection. This optimized system significantly reduces enzyme-linked immunosorbent assay (ELISA) time for faster disease diagnosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunotechnology

Background:

  • Matrix metalloproteinase 9 (MMP9) is implicated in inflammation, cancer invasion, metastasis, and angiogenesis.
  • Accurate and efficient detection of MMP9 is crucial for diagnosing related diseases.

Purpose of the Study:

  • To develop a rapid and sensitive detection system for MMP9.
  • To express a recombinant fragment antigen-binding (Fab)-type anti-MMP9 antibody.
  • To optimize the enzyme-linked immunosorbent assay (ELISA) protocol for MMP9 detection.

Main Methods:

  • Expression of recombinant anti-MMP9 Fab antibody in Escherichia coli.
  • Purification of the recombinant antibody.
  • Optimization of ELISA reaction conditions.
  • Determination of antigen-binding efficiency.

Main Results:

  • High-purity recombinant anti-MMP9 Fab antibody was obtained within five days.
  • The recombinant Fab demonstrated nanomolar-order antigen-binding efficiency.
  • Optimized ELISA protocol reduced reaction time from 20.5 hours to 3.5 hours.
  • A rapid and sensitive MMP9 detection system was established.

Conclusions:

  • The developed recombinant antibody and optimized ELISA provide a fast and sensitive method for MMP9 detection.
  • This system has potential applications in diagnosing inflammatory and cancer-related diseases associated with MMP9 overexpression.
Keywords:
Enzyme-linked immunosorbent assayEscherichia coli expressionMatrix metalloproteinase 9Point-of-care testingRecombinant fragment antigen-binding

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