Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

154
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
154
Amino Acid Catabolism01:18

Amino Acid Catabolism

120
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
120
Microbial Fermentation01:23

Microbial Fermentation

183
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
183
Bioremediation00:46

Bioremediation

19.8K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
19.8K
Lipid Catabolism01:25

Lipid Catabolism

143
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
143
Overview of Archaea01:29

Overview of Archaea

89
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
89

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rewiring a methanol-responsive regulatory system improves glucose-methanol co-utilization in Eubacterium limosum.

Journal of biological engineering·2026
Same author

Decoding multidimensional regulatory networks in <i>Streptomyces</i> to accelerate natural product discovery and production.

Natural product reports·2026
Same author

Adaptive laboratory evolution enhances methanol-driven CO<sub>2</sub> fixation in Sporomusa sphaeroides KIAC.

Bioresource technology·2026
Same author

A framework for building a synthetic cell from the SynCell Asia Initiative.

Nature biotechnology·2026
Same author

Facile fabrication of chitin-glucan nanofibers from four edible mushroom species using deep eutectic solvent and their adsorption characterization.

Food chemistry·2026
Same author

<b>Gut bacterial</b> <i><b>O</b></i><b>-demethylation modulates systemic exposure to oral etoposide</b>.

Gut microbes·2026

Related Experiment Video

Updated: Aug 23, 2025

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
15:28

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

14.6K

Recent progress in the engineering of C1-utilizing microbes.

Jiyun Bae1, Sangrak Jin1, Seulgi Kang1

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.

Current Opinion in Biotechnology
|November 5, 2022
PubMed
Summary
This summary is machine-generated.

Microbial conversion of one-carbon (C1) substrates offers a sustainable alternative to fossil fuels. Engineering microbes enhances their efficiency for industrial applications, paving the way for a low-carbon future.

More Related Videos

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.5K
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

3.9K

Related Experiment Videos

Last Updated: Aug 23, 2025

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
15:28

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Published on: November 16, 2012

14.6K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.5K
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

3.9K

Area of Science:

  • Biotechnology and Synthetic Biology
  • Sustainable Chemistry
  • Microbial Engineering

Background:

  • The global climate crisis necessitates sustainable alternatives to fossil fuel-based industries.
  • Microbial conversion of one-carbon (C1) substrates (e.g., CO2, methane, methanol) presents a promising low-carbon footprint solution.
  • Current C1-utilizing microbes have limitations in industrial scalability due to slow growth and low efficiency.

Purpose of the Study:

  • To review advancements in engineering C1-utilizing microbes for enhanced carbon assimilation.
  • To describe the creation of synthetic microorganisms by transferring C1 assimilation pathways.
  • To outline future research directions for industrial C1-utilizing microbial applications.

Main Methods:

  • Review of recent literature on microbial engineering for C1 substrate utilization.
  • Analysis of strategies for improving carbon assimilation efficiency in microbes.
  • Description of synthetic biology approaches for developing novel C1-utilizing microorganisms.

Main Results:

  • Significant progress has been made in enhancing the carbon assimilation efficiency of C1-utilizing microbes.
  • Synthetic microorganisms have been developed by introducing natural C1 assimilation pathways into non-C1-utilizing hosts.
  • Engineered microbes show potential for increased productivity and yield in C1 substrate conversion.

Conclusions:

  • Engineering and synthetic biology approaches are crucial for overcoming the limitations of natural C1-utilizing microbes.
  • Further research is needed to fully realize the industrial potential of these engineered microorganisms.
  • Microbial C1 utilization offers a viable pathway towards sustainable chemical and fuel production.