Comparison of aqueous humor ascorbic acid level in smokers and non-smokers

In this study, we studied effect of smoking on ascorbic acid level in aqueous humor. A cohort of 112 individuals undergoing cataract surgery for senile cataract (mean ± SD age-65 ± 8 years) was sub-grouped as smoker (n = 56) and non-smoker (n = 56) based on smoking habit. The aqueous humor sample was collected in beginning of the surgery and quantitative ascorbic acid estimation was done by colorimetric method (spectrophotometry at λ = 578 nm) using commercially available assay kits using the auto-analyzer assay procedure. The mean (±SD) aqueous humor ascorbic acid level was 1396 ± 629 μmol/L among non-smokers and 774 ± 436 μmol/L among smokers (p < 0.0001). The aqueous humor ascorbic acid concentration is significantly lower in smokers compared to non-smokers. The aqueous humor ascorbic acid concentration is affected by gender but not by age or morphology of cataract.