Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA as a Genetic Template02:05

DNA as a Genetic Template

22.5K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.5K
The DNA Helix01:16

The DNA Helix

140.5K
Overview
140.5K
Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K
Southern Blot02:57

Southern Blot

20.0K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
20.0K
DNA Isolation01:24

DNA Isolation

39.7K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
39.7K
Hardy-Weinberg Principle01:49

Hardy-Weinberg Principle

72.7K
Diploid organisms have two alleles of each gene, one from each parent, in their somatic cells. Therefore, each individual contributes two alleles to the gene pool of the population. The gene pool of a population is the sum of every allele of all genes within that population and has some degree of variation. Genetic variation is typically expressed as a relative frequency, which is the percentage of the total population that has a given allele, genotype or phenotype.
72.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel relationship between time offsets in capillary electrophoresis and DNA sequence variations in short tandem repeats.

Journal of forensic sciences·2026
Same author

Are genetically defined "metapopulations" self-evident in YHRD?

Forensic science international. Genetics·2026
Same author

Probabilistic searching of databases compared to binary CODIS eligibility: An improved methodology for maximizing investigative leads using short tandem repeat DNA profiles.

Forensic science international. Genetics·2026
Same author

Evaluation of the ForenSeq™ MainstAY workflow for forensic DNA samples.

Journal of forensic sciences·2026
Same author

Reporting the value of biological findings given activity level propositions: A discussion of published recommendations.

Journal of forensic sciences·2025
Same author

A murder in Boston.

Journal of forensic sciences·2025
Same journal

When termites mimic disease: Vertebral osteolysis and indoor scene alteration in the French West Indies.

Science & justice : journal of the Forensic Science Society·2026
Same journal

Forensic soil comparison for traffic accidents caused by soil clods on roadway.

Science & justice : journal of the Forensic Science Society·2026
Same journal

Chemical profiling of counterfeit perfumes: insights within a forensic intelligence approach.

Science & justice : journal of the Forensic Science Society·2026
Same journal

The forensic limits of hair drug testing: a critical assessment of drug intake and hair concentration relationships.

Science & justice : journal of the Forensic Science Society·2026
Same journal

Carrying the aftermath: forensic trauma as a predictable occupational injury in a lifecycle systems framework.

Science & justice : journal of the Forensic Science Society·2026
Same journal

Footprint analysis in a subadult Slovak population (aged 10-18 years) for stature estimation: a preliminary study.

Science & justice : journal of the Forensic Science Society·2026
See all related articles

Related Experiment Video

Updated: Aug 23, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.8K

Guiding proposition setting in forensic DNA interpretation.

John Buckleton1, Tim Kalafut2, James Curran3

  • 1Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland 1142, New Zealand; Department of Statistics, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.

Science & Justice : Journal of the Forensic Science Society
|November 6, 2022
PubMed
Summary
This summary is machine-generated.

Using conditioning profiles in DNA mixture analysis aids in distinguishing true donors. This study demonstrates a method to improve decision-making in complex cases, minimizing false inclusions.

Keywords:
ConditioningForensic DNA mixture interpretationProbabilistic genotyping

More Related Videos

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.5K
Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

34.8K

Related Experiment Videos

Last Updated: Aug 23, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.8K
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

20.5K
Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

34.8K

Area of Science:

  • Forensic Science
  • Genetics
  • Statistics

Background:

  • DNA mixture analysis presents challenges in donor identification.
  • A reluctance exists in using conditioning profiles for DNA mixtures.
  • Difficulties arise in specific scenarios, such as ambiguous prior probabilities.

Purpose of the Study:

  • To demonstrate the benefit of using conditioning profiles in DNA mixture analysis.
  • To provide guidance for complex decision-making in forensic casework.
  • To propose a method for calculating a lower bound on the Bayes factor.

Main Methods:

  • Utilizing rigorous mathematical treatment for decision-making.
  • Calculating a lower bound on the Bayes factor (BF).
  • Determining the highest likelihood ratio (LR) with and without the person of interest (POI) and disputed conditioning profile.

Main Results:

  • The proposed method provides a lower bound on the BF for POI inclusion.
  • This approach minimizes adventitious inclusions.
  • It approximates an exhaustive set of propositions for casework.

Conclusions:

  • Following mathematical guidance improves DNA mixture interpretation.
  • The method offers a robust approach for ambiguous prior probabilities in casework.
  • This technique enhances the reliability of forensic DNA evidence interpretation.