Lysosomal enzyme release from guinea pig alveolar cells: biochemical and morphologic observations

Cells lavaged from guinea pig lungs selectively release the lysosomal enzymes beta-glucuronidase and lysozyme during the process of phagocytosis. The total enzymatic activity of beta-glucuronidase was elevated in zymosan-exposed compared with non-exposed cells. The divalent cation carrying ionophore A23187 did not stimulate lysosomal enzyme release. Transmission and scanning electron micrographs demonstrate particle uptake and are consistent with enzyme release.