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Human Bone Marrow Mesenchymal Stem Cell (hBMSCs)-Derived miR-29a-3p-Containing Exosomes Impede Laryngocarcinoma Cell

Tingting Yu1, Hong Yu1, Dong Xiao1

  • 1Department of Otolaryngology, Head and Neck Surgery, The First Hospital, Jilin University, Changchun, Jilin, China.

Stem Cells International
|November 7, 2022
PubMed

Insights

Upregulated microRNA-29a-3p, delivered via mesenchymal stem cell exosomes, inhibits laryngocarcinoma progression by targeting PTEN. This discovery offers a potential new diagnostic and therapeutic strategy for laryngocarcinoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Laryngocarcinoma progression mechanisms are not fully understood.
  • The role of microRNA-29a-3p in laryngocarcinoma requires further elucidation.
  • Phosphatase and tensin homolog (PTEN) is a key tumor suppressor.

Purpose of the Study:

  • To investigate the inhibitory mechanisms of microRNA-29a-3p in laryngocarcinoma.
  • To explore the potential of human bone marrow mesenchymal stem cell (hBMSC)-derived exosomes as a delivery vehicle for microRNA-29a-3p.
  • To determine the relationship between microRNA-29a-3p and PTEN in laryngocarcinoma.

Main Methods:

  • Analysis of miR-29a-3p and PTEN levels in laryngocarcinoma tissues and cell lines.
  • Transfection of miR mimics/inhibitor into laryngocarcinoma cell lines (M4E, Hep2).
  • Isolation and characterization of exosomes from hBMSCs engineered to overexpress miR-29a-3p.
  • In vitro functional assays (proliferation, invasion, migration, apoptosis) and in vivo mouse models.

Main Results:

  • Laryngocarcinoma tissues and cells showed decreased miR-29a-3p and elevated PTEN levels.
  • miR-29a-3p mimics inhibited cancer cell proliferation, invasion, and migration, while promoting apoptosis.
  • hBMSC-derived exosomes enriched with miR-29a-3p suppressed laryngocarcinoma cell malignancy in vitro and tumor growth in vivo.
  • A direct binding interaction between miR-29a-3p and PTEN was confirmed.

Conclusions:

  • Upregulated miR-29a-3p delivered via hBMSC-derived exosomes inhibits laryngocarcinoma progression.
  • The mechanism involves the regulation of PTEN by miR-29a-3p.
  • This exosome-based miR-29a-3p delivery represents a promising therapeutic strategy for laryngocarcinoma.

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