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Related Experiment Video

Updated: Aug 22, 2025

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Reconstructing neural circuits using multiresolution correlated light and electron microscopy.

Karl Friedrichsen^1,2,3, Pratyush Ramakrishna^1,2,3, Jen-Chun Hsiang^1,2,3

¹Department of Ophthalmology and Visual Sciences, Washington University in St. Louis, St. Louis, MO, United States.

Frontiers in Neural Circuits

|November 7, 2022

Summary

This summary is machine-generated.

Multiresolution correlated light and electron microscopy (CLEM) efficiently targets electron microscopy imaging to optically characterized cells. This method links live optical imaging to detailed ultrastructural mapping for neuroscience research.

Keywords:

confocal 3D microscopy connectomics correlated light and electron microscopy (CLEM)electron microscopy neural circuit synapse tissue mapping

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Last Updated: Aug 22, 2025

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

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Area of Science:

Neuroscience
Microscopy
Cell Biology

Background:

Correlated light and electron microscopy (CLEM) integrates functional neuronal data with synaptic anatomy.
High-throughput electron microscopy (EM) requires efficient targeting of specific cells.

Purpose of the Study:

To develop and demonstrate a multiresolution approach to CLEM (mrCLEM).
To enable efficient targeting of EM imaging to optically characterized cells.
To maintain optimal tissue preparation for high-throughput EM reconstruction.

Main Methods:

Multiresolution imaging using scanning electron microscopy (SEM) on section arrays.
Multiresolution confocal mapping of aldehyde-fixed tissue.
Iterative feature matching from gross anatomy to subcellular structures for targeted EM acquisition.

Main Results:

Demonstrated correspondence between low-resolution EM and optical maps.
Successful iterative feature matching for high-resolution EM targeting.
Provided examples from mouse retinal and brain slice experiments linking light and EM imaging.

Conclusions:

mrCLEM efficiently targets EM imaging to specific cells identified by optical microscopy.
The approach facilitates high-throughput EM reconstruction by optimizing tissue preparation and targeting.
This technique bridges live optical imaging with detailed ultrastructural analysis in neuroscience.