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Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

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Approaches for evaluation of novel CPP-based cargo delivery systems.

Ly Porosk¹, Ülo Langel^1,2

¹Laboratory of Drug Delivery, Institute of Technology, Faculty of Science and Technology, University of Tartu, Tartu, Estonia.

Frontiers in Pharmacology

|November 7, 2022

Summary

This summary is machine-generated.

Cell-penetrating peptides (CPPs) efficiently deliver cargo across cell membranes. This review details diverse in silico, in vitro, and in vivo methods for evaluating CPP delivery systems.

Keywords:

cargo delivery cell-penetrating peptides evaluation approaches nucleic acid therapeutics transfection

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Area of Science:

Biochemistry and Molecular Biology
Drug Delivery Systems
Peptide Science

Background:

Cell-penetrating peptides (CPPs) are short peptides capable of crossing cell membranes.
CPPs facilitate the transport of various cargoes, including drugs and nucleic acids.
Effective evaluation of CPP delivery systems is crucial for successful applications.

Purpose of the Study:

To review and summarize diverse evaluation methods for CPP-based cargo delivery systems.
To highlight the importance of matching evaluation techniques to CPP delivery characteristics.
To provide a comprehensive overview of in silico, in vitro, and in vivo approaches.

Main Methods:

Literature review of existing research on CPP evaluation.
Categorization of methods into in silico, in vitro, and in vivo approaches.
Discussion of techniques including fluorophore attachment and multi-disciplinary tools.

Main Results:

Multiple methods exist for evaluating CPP efficacies, with fluorophore attachment being common.
Successful CPP delivery system assessment requires methods tailored to specific delivery characteristics.
A combination of chemistry, physics, molecular biology, and engineering tools are applied.

Conclusions:

Diverse in silico, in vitro, and in vivo approaches are available for CPP evaluation.
The choice of method significantly impacts the assessment of CPP-cargo systems.
This review consolidates current methodologies for characterizing CPP delivery systems.