Nitrogen fertilization and CO₂ concentration synergistically affect the growth and protein content of Agropyron...

The nitrogen (N) and protein concentrations in plant tissues exposed to elevated CO₂ (eCO₂) generally decline , such declines in forage grass composition are expected to have negative implications for the nutritional and economic value of grass. Plants require N for the production of a photosynthetically active canopy and storage proteins in the tissues, whose functionality will strongly influence productivity and quality. The objective of this study was to investigate whether eCO₂ plus N-fertilization increases growth and N nutrition of Agropyron mongolicum, and the dependence of this improvement on the coordination between root and leaf development.

We analyzed A. mongolicum from field-grown within the open-top chambers (OTCs) facility under two atmospheric CO₂ (ambient, 400 ± 20 µmol mol^-1, aCO₂, and elevated, 800 ± 20 µmol mol^-1, eCO₂) and three N-fertigation treatments (control, low N-fertigation , and high N-fertigation) for two months.

Elevated CO₂ plus N-fertigation strongly increased shoot and root biomass, and the nitrogen and protein concentrations of A. mongolicum compared to those plants at aCO₂ levels. Increased N content in leaves and reduced specific leaf area (SLA) at a high N supply could alleviate photosynthetic acclimation to eCO₂ and drive the production of greater shoot biomass with the potential for higher photosynthesis, productivity, and nutritional quality. The increased root length (RL), the ratio of total aboveground N taken up per RL (TN/RL), stomatal conductance (Gs), and transpiration rate (Tr) contribute to the transpiration-driven mass flow of N, consequently increasing N uptake by roots. In addition, a smaller percentage of N remained as unassimilated nitrate ( $$ ) under eCO₂, indicating that assimilation of $$ into proteins was not inhibited by eCO₂. These findings imply that grass productivity and quality will enhance under anticipated elevated CO₂ concentration when effective management measures of N-fertilization are employed.