Hsa_circ_0097271 Knockdown Attenuates Osteosarcoma Progression via Regulating miR-640/MCAM Pathway

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Updated: Aug 22, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Peng Sun1, Xuan Yang1

  • 1Joint Surgery, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, 430081 Hubei, China.

Disease Markers
|November 7, 2022
PubMed
Abstract

Insights

Circular RNA circ_0097271 promotes osteosarcoma (OS) development by targeting the miR-640/MCAM pathway. Downregulating circ_0097271 inhibits OS cell growth and tumor progression, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in the malignant progression of various cancers, including osteosarcoma (OS).
  • The specific role and mechanism of circ_0097271 in OS development have not been fully elucidated.
  • Understanding circ_0097271's function is crucial for developing novel therapeutic strategies for OS.

Purpose of the Study:

  • To investigate the functional role of circ_0097271 in osteosarcoma (OS) development.
  • To elucidate the underlying molecular mechanism of circ_0097271 in OS progression.
  • To explore circ_0097271 as a potential therapeutic target for OS treatment.

Main Methods:

  • Quantitative real-time PCR (qPCR) to analyze the expression of circ_0097271, miR-640, and MCAM in OS tissues and cells.
  • Cellular assays (CCK-8, colony formation, Transwell) to assess the impact of circ_0097271 on OS cell proliferation and migration.
  • In vivo animal models to evaluate the effect of circ_0097271 on tumor growth.
  • Dual-luciferase reporter and RIP assays to confirm the binding interactions between circ_0097271, miR-640, and MCAM.

Main Results:

  • Circ_0097271 expression was significantly upregulated in OS samples and cells.
  • Knockdown of circ_0097271 inhibited OS cell proliferation, migration, and in vivo tumor growth.
  • Circ_0097271 acted as a sponge for miR-640, leading to decreased miR-640 levels. MiR-640 targeted MCAM, suppressing its expression.
  • MCAM knockdown mimicked the inhibitory effects of circ_0097271 knockdown, and miR-640 depletion partially reversed these effects.

Conclusions:

  • Circ_0097271 functions as an oncogenic driver in osteosarcoma.
  • The circ_0097271/miR-640/MCAM axis plays a critical role in OS development and progression.
  • Circ_0097271 represents a promising therapeutic target for osteosarcoma treatment.

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