Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Photonic arbitrary Chern vectors.

Science advances·2026

Same author

Cyclin K condensates bridge CDK12 to phosphorylate and drive oncogenic YAP activation in hepatocellular carcinoma.

Science advances·2026

Same author

Spatiotemporal Graph Learning on Urban Environments.

Environmental science & technology·2026

Same author

Radioluminescent glass and fibers for neutron detection.

Materials horizons·2026

Same author

Applicability of machine learning in predicting N<sub>2</sub>O emission from wastewater treatment processes: a narrative review.

Journal of environmental management·2026

Same author

Deficiency of the voltage‑gated proton channel Hv1 aggravates lung damage induced by exposure to carboxylated carbon black and carboxylated carbon black@lead.

Toxicological research·2026

Search research articles

Related Experiment Video

Updated: Aug 22, 2025

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Expression patterns and biological function of BCL2L10 during mouse preimplantation development.

Yong Liu¹, Jing Xin², Shengnan Zhang²

¹Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, 236037, China; Department of Veterinary and Animal Sciences, Animal Models Core Facility, Institute for Applied Life Sciences (IALS), University of Massachusetts Amherst, Amherst, MA, 01002, United States.

Gene Expression Patterns : GEP

|November 7, 2022

Summary

BCL2-like 10 (BCL2L10) is vital for mouse oocyte meiosis and early embryonic development. Its depletion impairs blastocyst formation and reduces key developmental gene expression, highlighting its role in developmental competence.

Keywords:

BCL2L10 Developmental competence Expression patterns Mouse embryo Preimplantation

More Related Videos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

Related Experiment Videos

Last Updated: Aug 22, 2025

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Published on: April 12, 2013

Area of Science:

Developmental Biology
Cell Biology
Reproductive Biology

Background:

BCL2-like 10 (BCL2L10) is known for its role in oocyte meiosis.
Its function during preimplantation embryonic development is not well understood.

Purpose of the Study:

To investigate the expression patterns of Bcl2l10 during mouse preimplantation development.
To determine the biological function of BCL2L10 in early embryogenesis.

Main Methods:

RT-qPCR and immunofluorescence were used to analyze Bcl2l10 expression.
siRNA and morpholino were employed to knock down Bcl2l10 in pronuclear embryos.

Main Results:

Bcl2l10 expression was high in MII oocytes and pronuclear embryos, decreasing significantly by the two-cell stage.
BCL2L10 protein was detected in cytoplasm and nuclei throughout preimplantation development.
BCL2L10 knockdown reduced blastocyst formation rates and decreased OCT4, NANOG, and SOX17 expression.

Conclusions:

BCL2L10 plays a critical role in the developmental competence of preimplantation mouse embryos.
Its expression dynamics and function are essential for successful early embryonic development.