Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Heterogeneous Reactivity of Palladium Nanoparticles Revealed by Wavelength-Resolved Interferometric Scattering.

Nano letters·2026

Same author

Dynamic Interfacial Processes in Single Hydrogen Microbubbles Probed by Operando Opto-Electrochemistry.

Analytical chemistry·2026

Same author

Peer Review and AI: Your (Human) Opinion Is What Matters.

ACS nano·2026

Same author

Kinetically Controlled Seed-Mediated Synthesis of Colloidal Copper Nanotetrahedra with Intricate Internal Structure.

Journal of the American Chemical Society·2025

Same author

The Next Ten Years of Nanochemistry: Summary of a Community Workshop on Key Challenges and Opportunities.

ACS nano·2025

Same author

Learning from Metal Nanocrystal Heterogeneity: A Need for Information-Rich and High-Throughput Single-Nanocrystal Measurements.

ACS nanoscience Au·2025

Same journal

Real-Time Vibrational Spectroscopy Reveals an Inversion Transition State in the Photoisomerization of Phenylazoimidazole.

The journal of physical chemistry letters·2026

Same journal

Precursor-Directed Self-Assembly in Hydrothermal Carbon Nitride Nanostructures Revealed by Nano-FTIR.

The journal of physical chemistry letters·2026

Same journal

Correction to "Equation-of-Motion Block-Correlated Coupled Cluster Method for Excited Electronic States of Strongly Correlated Systems".

The journal of physical chemistry letters·2026

Same journal

Rationalizing Stacking-Dependent Charge Injection Dynamics in Radical-Based Organic Light-Emitting Diodes.

The journal of physical chemistry letters·2026

Same journal

Bottom-Up Formation of the Simplest Geminal Thiol─Methanedithiol (CH<sub>2</sub>(SH)<sub>2</sub>)─and the Methyl Hydrodisulfide (H<sub>3</sub>CSSH) Isomer in Interstellar Analogue Ices.

The journal of physical chemistry letters·2026

Same journal

Trion Mediated Sequential Charge Separation in Functionalized CsPbBr<sub>3</sub>/AgInS<sub>2</sub> Hybrid Nanocrystals.

The journal of physical chemistry letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 22, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Calcite-Assisted Localization and Kinetics (CLocK) Microscopy.

Joseph W Monaghan¹, Zachary J O'Dell¹, Sanjay Sridhar¹

¹Department of Chemistry, Temple University, Philadelphia, Pennsylvania19122, United States.

The Journal of Physical Chemistry Letters

|November 7, 2022

Summary

This summary is machine-generated.

Calcite-assisted localization and kinetics (CLocK) microscopy offers multiparameter super-resolution imaging, enabling <10 nm resolution with polarization, orientation, and kinetic information. This technique provides new mechanistic insights into dynamic processes at the nanoscale.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Related Experiment Videos

Last Updated: Aug 22, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Area of Science:

Optical Microscopy
Nanoscale Imaging
Biophysics

Background:

Super-resolution microscopy techniques surpass the diffraction limit for enhanced spatial resolution.
Encoding additional information like anisotropy and orientation into super-resolved images remains a challenge.

Purpose of the Study:

Introduce calcite-assisted localization and kinetics (CLocK) microscopy.
Enable multiparameter super-resolution imaging with polarization, orientation, and kinetic information.
Provide mechanistic insight into dynamic nanoscale processes.

Main Methods:

Integration of a rotating calcite crystal into standard optical microscope setups.
Utilizing the unique point spread function of CLocK images for data encoding.
Achieving emitter/scatterer localization with <10 nm resolution.

Main Results:

CLocK microscopy provides immediate polarization and orientation data.
Enables localization with sub-10 nm spatial resolution.
Captures kinetic information faster than camera integration times.

Conclusions:

CLocK microscopy is a cost-effective and easily integrated technique for advanced super-resolution imaging.
It offers a powerful new tool for studying dynamic nanoscale phenomena.
Facilitates mechanistic understanding of processes like nanoparticle dissolution and SERS.