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Related Experiment Video

Updated: Aug 22, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Selecting Better Biocatalysts by Complementing Recoded Bacteria.

Rudy Rubini¹, Suzanne C Jansen¹, Houdijn Beekhuis¹

¹Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.

Angewandte Chemie (International Ed. in English)

|November 7, 2022

Summary

This summary is machine-generated.

This study introduces a novel in vivo selection method for enzyme evolution using recoded organisms. This approach enhances biocatalyst development by linking non-canonical amino acid production to cellular survival, enabling significant efficiency improvements.

Keywords:

Carbamoylases Directed Evolution Genetic-Code Expansion In Vivo Selection Synthetic Biology

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Last Updated: Aug 22, 2025

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Published on: October 31, 2019

Area of Science:

Biotechnology
Synthetic Biology
Enzyme Engineering

Background:

In vivo selections are crucial for directed enzyme evolution but challenging for non-metabolic enzymes.
Linking enzymatic activity to cellular survival is a key limitation.

Purpose of the Study:

To develop a versatile in vivo selection strategy for evolving biocatalysts.
To engineer enzymes providing non-canonical amino acids (ncAAs) from synthetic precursors.

Main Methods:

Utilized recoded organisms dependent on ncAAs.
Employed serial passaging under selective conditions.
Engineered carbamoylases for ncAA precursor conversion.

Main Results:

Achieved catalytic efficiencies over five orders of magnitude higher than wild-type enzymes.
Demonstrated correlation between bacterial growth rates and enzymatic activity.
Successfully evolved improved enzyme variants with minimal intervention.

Conclusions:

The developed platform enables efficient in vivo directed evolution of biocatalysts.
The strategy is versatile, requiring minimal human intervention and no specialized equipment.
This method facilitates the evolution of enzymes for producing essential building blocks like ncAAs.