Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales.

bioRxiv : the preprint server for biology·2026

Same author

[Clinical efficacy and safety of CLAE regimen for refractory/relapsed T cell acute lymphoblastic leukemia/lymphoma: a prospective, multicenter, single-arm study].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025

Same author

Retraction notice to "P19 embryonal carcinoma cells as in vitro model for studying purinergic receptor expression and modulation of NMDA-glutamate and acetylcholine receptors during neuronal differentiation". [Neuroscience 146 (2007) 1169-1181].

Neuroscience·2025

Same author

Utility of long-read sequencing for All of Us.

Nature communications·2024

Same author

[Clinical features of children with <i>Cunninghamella</i> spp. infection: a case report and literature review].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2022

Same author

[A three-series case report and literature review of acute myeloid leukemia with t(7;11)(p15;p15)/NUP98-HOXA9].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2022

Search research articles

Related Experiment Video

Updated: Aug 22, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Multishot tomography for high-resolution in situ subtomogram averaging.

S Khavnekar¹, W Wan², P Majumder¹

¹MPI for Biochemistry, Germany.

Journal of Structural Biology

|November 7, 2022

Summary

This summary is machine-generated.

We developed a fast cryo-electron tomography (cryo-ET) method for faster in situ subtomogram averaging (STA). This technique improves throughput for observing macromolecules within their native cellular context.

More Related Videos

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

Related Experiment Videos

Last Updated: Aug 22, 2025

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

Area of Science:

Structural Biology
Biophysics
Microscopy

Background:

Cryo-electron tomography (cryo-ET) enables high-resolution imaging of protein complexes.
Subtomogram averaging (STA) refines cryo-ET data for near-atomic resolution.
Focused ion beam (FIB) milling allows in situ observation of macromolecules within native cellular environments.

Purpose of the Study:

To address the slow acquisition speed of traditional cryo-ET, which limits project throughput.
To develop an accelerated data acquisition strategy for in situ STA.
To enable faster structural analysis of macromolecular complexes in their native cellular context.

Main Methods:

Implementation of a fast, multi-position tomographic acquisition scheme.
Utilizing beam-tilt corrected beam-shift imaging along the tilt axis.
Application of subtomogram averaging to processed cryo-ET data.

Main Results:

Achieved sub-nanometer resolution for in situ STA averages.
Demonstrated a significant increase in overall project throughput compared to conventional cryo-ET methods.
Successfully visualized macromolecular complexes within their native cellular environment with improved speed.

Conclusions:

The proposed fast acquisition scheme enhances the efficiency of cryo-ET for in situ structural studies.
This method accelerates the process of obtaining high-resolution structural information of cellular components.
It opens new possibilities for studying dynamic biological processes at the molecular level in situ.