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Updated: Aug 22, 2025

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

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Published on: October 27, 2020

Hippocampal astrocytes modulate anxiety-like behavior.

Woo-Hyun Cho¹, Kyungchul Noh², Byung Hun Lee³

¹Department of Physiology and Neuroscience, Dental Research Institute, Seoul National University School of Dentistry, Seoul, 08826, Republic of Korea. woohyuncho15@snu.ac.kr.

Nature Communications

|November 7, 2022

Summary

Hippocampal astrocytes regulate mouse anxiety by releasing ATP, enhancing synaptic transmission. Activating these astrocytes reduces anxiety-like behaviors, suggesting a therapeutic target for anxiety disorders.

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Last Updated: Aug 22, 2025

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Published on: May 26, 2023

Area of Science:

Neuroscience
Astrocytes biology
Behavioral neuroscience

Background:

Astrocytes modulate synaptic transmission, but their role in affective behavior is unclear.
Understanding astrocyte influence on anxiety is crucial for developing new treatments.

Purpose of the Study:

To investigate the role of hippocampal astrocytes in modulating anxiety-like behaviors in mice.
To explore the underlying mechanisms of astrocyte-mediated anxiety regulation.

Main Methods:

In vivo two-photon calcium imaging to monitor astrocyte activity during behavioral tests.
Optogenetic activation of hippocampal astrocytes in hGFAP-ChR2 transgenic mice.
Measurement of synaptic transmission in the dentate gyrus.

Main Results:

Hippocampal astrocyte calcium activity correlated with mouse affective state.
Optogenetic activation of astrocytes induced anxiolytic behaviors.
ATP release from activated astrocytes enhanced excitatory synaptic transmission in dentate gyrus granule cells.

Conclusions:

Hippocampal astrocytes play a key role in modulating anxiety-like behaviors.
ATP-mediated regulation of synaptic homeostasis in the dentate gyrus is a critical mechanism.
Targeting hippocampal astrocytes offers a potential therapeutic strategy for anxiety disorders.