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Integrated genomics and proteomics analysis of Paenibacillus peoriae IBSD35 and insights into its antimicrobial

Ng Ngashangva1, Pulok K Mukherjee1, Chandradev Sharma1

  • 1A National Institute of Department of Biotechnology, Institute of Bioresources and Sustainable Development (IBSD), Govt. of India, Takyelpat, Imphal, Manipur, 795001, India.

Scientific Reports
|November 7, 2022
PubMed

Insights

New antimicrobial peptides from Paenibacillus peoriae IBSD35 show broad-spectrum activity against resistant microbes. This discovery offers potential for novel antimicrobial agents to combat antimicrobial resistance (AMR).

Area of Science:

  • Microbiology
  • Biochemistry
  • Genomics

Background:

  • Antimicrobial resistance (AMR) is a growing global health threat requiring novel therapeutic strategies.
  • Naturally occurring antimicrobial peptides (AMPs) present a promising alternative due to low resistance development.
  • Paenibacillus peoriae IBSD35 was investigated as a source of potent antimicrobial compounds.

Purpose of the Study:

  • To isolate and characterize antimicrobial peptides from Paenibacillus peoriae IBSD35.
  • To identify the biosynthetic gene clusters responsible for AMP production.
  • To explore the potential of these AMPs as novel agents against AMR.

Main Methods:

  • Cell-free supernatant from P. peoriae IBSD35 was processed using salting out and chromatography.
  • Purified peptides were analyzed using liquid chromatography-tandem-mass spectrometry (LC-MS/MS).
  • Genomic and proteomic data were integrated to predict biosynthetic gene clusters.

Main Results:

  • The purified extract demonstrated high, broad-spectrum antimicrobial activity.
  • LC-MS/MS identified specific peptides, which were linked to their encoding genes via genomic data.
  • P. peoriae IBSD35 possesses genes for endophytic traits and nonribosomal peptide synthetases.

Conclusions:

  • Paenibacillus peoriae IBSD35 is a viable source for discovering novel antimicrobial peptides.
  • Integrated genomics and proteomics facilitate the identification of AMP biosynthetic pathways.
  • This research enhances the potential for developing new bio-molecules to combat antimicrobial resistance.

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