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Zero-Interfacing μHPLC to ICPMS.

Yang Zhou1, Xingrui Song1, Xiaowen Yan1

  • 1Department of Chemistry & the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen361005, China.

Analytical Chemistry
|November 8, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a zero-interfacing method to couple microbore High-Performance Liquid Chromatography (HPLC) with Inductively Coupled Plasma Mass Spectrometry (ICPMS). This approach minimizes peak broadening, improving quantification of trace analytes in complex biosamples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectrometry

Background:

  • Chromatography-mass spectrometry is vital for trace analyte quantification in biosamples.
  • Peak broadening and remixing in hyphenated techniques reduce analytical accuracy.
  • Dead-volume in interfaces causes molecular diffusion, compromising resolution.

Purpose of the Study:

  • To develop a zero-interfacing approach for coupling microbore High-Performance Liquid Chromatography (μHPLC) with Inductively Coupled Plasma Mass Spectrometry (ICPMS).
  • To overcome peak broadening issues in hyphenated chromatography-mass spectrometry systems.
  • To improve the accurate determination of heteroatoms and their binding biomolecules.

Main Methods:

  • A novel column-nebulizer (COL-NEB) assembly was designed using a glass framework with a tapered nozzle.
  • The COL-NEB harbors a capillary chromatographic column and facilitates online nebulization via an Ar gas flow.
  • This setup directly interfaces μHPLC eluent into the ICPMS central sampling channel, minimizing dead-volume.

Main Results:

  • Achieved significant reduction in chromatographic peak width (e.g., 1.71 ± 0.07 s for a SLUGT peptide).
  • Demonstrated a low limit of detection (LOD) of 0.72 fg for 80Se.
  • Successfully determined over 32 Se-containing peptides in yeast lysate within 10 minutes, a record for this method.

Conclusions:

  • The zero-interfacing approach preserves chromatographic resolution and ICPMS detection limits.
  • This method enhances the accurate quantification of trace heteroatoms and associated biomolecules.
  • The developed technique offers a promising pathway for detailed biological analysis.