New bisbenzylisoquinoline alkaloid, isolation and structure elucidation from Cocculus pendulus

Area of Science:

• Phytochemistry
• Natural Products Chemistry
• Organic Chemistry

Background:

• Cocculus pendulus is a plant species with potential medicinal properties.
• Previous research has identified various alkaloids from the Cocculus genus.

Purpose of the Study:

• To conduct phytochemical investigations on the aerial parts of Cocculus pendulus.
• To isolate and identify chemical constituents from the plant.
• To elucidate the structure of novel compounds and confirm their identity.

Main Methods:

• Phytochemical screening of Cocculus pendulus aerial parts.
• Isolation and purification of compounds using chromatographic techniques.
• Structure elucidation using 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry.
• Chemical synthesis to confirm the structure of the new compound.

Main Results:

• Identification of a new compound, 2N-methylkohatine (1).
• Isolation of four known compounds: Kohatine (2), 1,2-Dehydrokohatine (3), and 5'Hydroxyapateline (4).
• The structure of 2N-methylkohatine was confirmed by its synthesis from Kohatine.

Conclusions:

• The study successfully identified and characterized a new alkaloid, 2N-methylkohatine, from Cocculus pendulus.
• The findings contribute to the understanding of the chemical diversity of Cocculus species.
• The synthesis of the new compound validates its structural assignment.