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Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

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AIEgens: Next Generation Signaling Source for Immunoassays?

Leina Dou¹, Qing Li¹, Zhanhui Wang¹

¹Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.

|November 8, 2022

Summary

Aggregation-induced emission (AIEgens) enhance immunoassays for sensitive detection. This review covers AIEgens probes, working principles, and applications in diagnostics and monitoring, guiding future development.

Keywords:

AIEgens aggregation-induced emission emission mechanism fluorescence fluorescent immunolabels fluorogenic substrates immunoassays signal transduction

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Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Aggregation-induced emission luminogens (AIEgens) offer unique photophysical properties.
AIEgens have emerged as promising tools for enhancing immunoassay performance.
Existing literature lacks comprehensive reviews on AIEgens-based immunoassays.

Purpose of the Study:

To provide a comprehensive review of AIEgens-based immunoassays.
To elucidate the working principles, probe structures, and mechanisms of AIEgens in immunoassays.
To explore applications and future directions in this rapidly evolving field.

Main Methods:

Literature review of AIEgens-based immunoassays.
Analysis of AIEgens structures and emission mechanisms.
Exploration of immunoassay platforms and applications.

Main Results:

AIEgens offer high sensitivity, specificity, and reliability in immunoassays.
Applications span food safety, medical diagnostics, and environmental monitoring.
Key elements include AIEgen structure, emission mechanism, and assay platform.

Conclusions:

AIEgens-based immunoassays represent a significant advancement in analytical techniques.
Further improvements in AIEgen performance and assay design are anticipated.
This review provides guidelines for selecting appropriate AIEgens-based immunoassays.