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Updated: Aug 22, 2025

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
Published on: October 4, 2024
Study of spatiotemporal regulation of kinase signaling using genetically encodable molecular tools
Danielle L Schmitt1, Sohum Mehta2, Jin Zhang3
1Department of Pharmacology, University of California San Diego, USA; Department of Chemistry and Biochemistry, University of California Los Angeles, USA.
Abstract:
Precise spatiotemporal organization and regulation of signal transduction networks are essential for cellular response to internal and external cues. To understand how this biochemical activity architecture impacts cellular function, many genetically encodable tools which regulate kinase activity at a subcellular level have been developed. In this review, we highlight various types of genetically encodable molecular tools, including tools to regulate endogenous kinase activity and biorthogonal techniques to perturb kinase activity. Finally, we emphasize the use of these tools alongside biosensors for kinase activity to measure and perturb kinase activity in real time for a better understanding of the cellular biochemical activity architecture.
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