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Related Experiment Video

Updated: Aug 22, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Current strategies for SARS-CoV-2 molecular detection.

Lei Xie¹, Junlin Li¹, Ying Ai²

¹Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou 510080, China. 303877469@qq.com.

Analytical Methods : Advancing Methods and Applications

|November 9, 2022

Summary

Accurate SARS-CoV-2 detection is crucial for pandemic control. Combining antibody tests with quantitative PCR (Q-PCR) and improving variant detection methods can enhance screening efficiency and reduce costs.

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Last Updated: Aug 22, 2025

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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

Area of Science:

Molecular biology
Virology
Diagnostic technologies

Background:

Accurate molecular detection of SARS-CoV-2 is vital for controlling pandemic spread.
Sample selection and testing strategies are critical for effective SARS-CoV-2 screening.
Quantitative PCR (Q-PCR) is the primary method, but challenges remain with cost, sample availability, and emerging variants.

Purpose of the Study:

To review current strategies for SARS-CoV-2 molecular detection.
To highlight the importance of sample selection and combined detection methods.
To identify areas for future research in improving SARS-CoV-2 diagnostics.

Main Methods:

Review of nucleotide amplification assays, including Q-PCR and isothermal amplification.
Analysis of antibody-based detection strategies in conjunction with Q-PCR.
Examination of pooled testing and multi-target assays for variant detection.

Main Results:

Q-PCR remains the dominant SARS-CoV-2 detection assay despite limitations.
Antibody-based detection combined with Q-PCR can increase positive case identification.
Pooled testing enhances efficiency and reduces costs for large-scale screening.
Multi-target assays are effective for detecting mutated SARS-CoV-2 variants.

Conclusions:

Future research should focus on optimizing sample collection strategies.
Enhancing Q-PCR primers and probes for mutated variants is essential.
Developing more economical isothermal amplification assays is needed.
Advanced antibody/antigen strategies require further exploration for improved diagnostics.