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Related Experiment Video

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Area-based Image Analysis Algorithm for Quantification of Macrophage-fibroblast Cocultures
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Topology-based fluorescence image analysis for automated cell identification and segmentation.

Luca Panconi1, Maria Makarova2, Eleanor R Lambert1

  • 1Institute of Immunology and Immunotherapy, School of Mathematics and Centre of Membrane Proteins and Receptors, University of Birmingham, Birmingham, UK.

Journal of Biophotonics
|November 9, 2022
PubMed
Summary

We developed TOBLERONE, a novel topological image analysis tool for accurate and objective cell segmentation. This method automates the quantification of plasma membrane order using confocal microscopy images.

Keywords:
biologyconfocal microscopydata analysisdyeslipidplasma membrane

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Area of Science:

  • Computational Biology
  • Image Analysis
  • Cell Biology

Background:

  • Manual cell segmentation is time-consuming and subjective.
  • Accurate cell segmentation is crucial for extracting biological information.
  • Quantifying plasma membrane order is important for understanding cell function.

Purpose of the Study:

  • To introduce Topological Boundary Line Estimation using Recurrence Of Neighbouring Emissions (TOBLERONE) for automated cell segmentation.
  • To demonstrate the utility of topological data analysis in image analysis.
  • To automate the quantification of plasma membrane order.

Main Methods:

  • Developed TOBLERONE, a topological image analysis tool.
  • Employed persistent homology to identify image features.
  • Utilized confocal microscopy images with environmentally-sensitive dyes.

Main Results:

  • TOBLERONE provides accurate segmentation of arbitrarily-shaped cells.
  • Topological data analysis offers objective data extraction.
  • The tool successfully automates the quantification of plasma membrane order.

Conclusions:

  • TOBLERONE enables automatic and objective cell segmentation.
  • Topological analysis is a powerful alternative to geometric methods for image analysis.
  • This tool facilitates the study of plasma membrane order in various cell types.