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Updated: Aug 22, 2025
Polymeric Microneedle Array Fabrication by Photolithography
Published on: November 17, 2015
Sometimes faster can be better: Microneedling IPG strips enables higher throughput for integrative top-down
Katrina Carbonara1, Jens R Coorssen2,3
1Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, St. Catharines, Ontario, Canada.
Abstract:
Passive rehydration of immobilized pH gradient (IPG) strips for two-dimensional gel electrophoresis (2DE) has, to our knowledge, never been quantitatively evaluated to determine an ideal rehydration time. Seeking to increase throughput without sacrificing analytical rigor, we report that a substantially shorter rehydration time is accomplished when surface area of IPG strips is increased via microneedling. Rehydration for 4 h, post microneedling, provides comparable results to overnight rehydration in final analyses by 2DE, while also shortening the overall protocol by 1 day.
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